将细菌的lacZ基因插入,使其由内源性基因驱动β-半乳糖苷酶的表达。RT-PCR分析显示,除了胰腺外,大多数检测的组织中都存在基因转录;β-半乳糖苷酶在大脑、眼睛、外耳和男性生殖系统中可检测到表达。 (Source: The inserted bacterial lacZ gene is expressed under the endogenous promoter, with detectable beta-galactosidase expression in brain, eyes, external ear, and male reproductive system by RT-PCR analysis, except for the pancreas.)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count