通过同源重组,编码区1和2被靶向。通过RT-PCR和 Western blot 分析,没有在纯合突变大脑组织中检测到转录物或蛋白质。(来源:J:81988, J:103485)
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Coding exons 1 and 2 were targeted by homologous recombination. Neither transcript nor protein was detected in homozygous mutant brain tissue by RT-PCR and Western blot analyses. (J:81988, J:103485)