Map3k4^tm1Glj

Two mutations were inserted into exon 21: a silent mutation in codon 1357 from CTG to CTC, and an AAG to AGG in 1361 that produced the replacement of the active site lysine by an arginine. Western blot of mutant MEFs demonstrated the expression of protein was comparable to wild-type, and inactivation of the kinase activity was validated in vivo. (J:101350)