这种靶向构建将2号和3号外显子替换为诺卡因抗性基因座,并导致了框架移位。 Western blot结果显示,纯合小鼠中没有检测到 caspase 7 的表达。(来源:J:105496)
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The targeting construct replaces exons 2 and 3 with a neomycin resistance cassette, and also results in a frame-shift. Western blot analysis showed absence of caspase 7 expression in homozygous mice. (J:105496)