一种新霉素抗性基因通过同源重组替换掉了四个编码外显子。RT-PCR在突变骨髓间质成骨细胞中未能检测到转录物,而 Western blot 证实了突变体中没有蛋白质存在。(来源:J:100858)
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A neomycin resistance gene replaced the four coding exons via homologous recombination. RT-PCR failed to detect transcript in mutant bone marrow stromal osteoblasts, and western blot confirmed absence of protein in mutants. (J:100858)