在exon 1的ATG位置插入了EGFP。载体中包含了一个loxP位点修饰的neo。 Western blot结果显示,这些突变体相较于neo去除时,大脑皮层中的GFP含量较少。(来源:J:86389, J:144883)
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An EGFP was inserted at the ATG in exon 1. A loxP-flanked neo was included in the vector. Western blot demonstrated that these mutants had less GFP in the cerebral cortex than when the neo was removed. (J:86389, J:144883)