将1-3号外显子替换为nLacZ载体。与小鼠中携带Cre的生殖细胞发生交配,移除了靶向载体中含有的 floxed neo。突变胸腺、肝脏和脾脏的 Western blot 检测并未发现蛋白质。(来源:J:100807)
查看原文 参与反馈
Exons 1-3 were replaced with an nLacZ cassette. Crossing with mice expressing cre in the germline removed a floxed neo included in the targeting vector. Western blot of mutant thymus, liver and spleen failed to detect protein. (J:100807)