整个编码序列被替换了为抗氨苄青霉素抗性 cassette, Western blot 证实了对突变体的有效靶向以及翻译的缺失。(来源:J:100408)
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The entire coding sequence was replaced with a neomycin resistance cassette. Western blot confirmed successful targeting in mutants and ablation of translation. (J:100408)