在第3外显子插入了新霉素抗性基因座。这些突变体产生了两种异常转录本,其中前半段exon 3被删除。 Western blot结果显示这些转录本并未翻译成蛋白质。(来源:J:100181)
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A neomycin resistance cassette was inserted into exon 3. Mutants produced two aberrant transcripts in which the first half of exon 3 was deleted. Western blot revealed that these transcripts were not translated into protein. (J:100181)