设计了一个靶向元件,用于将PGK-neo插入到exon 1,从而消除编码序列的92%,并通过 Western blot 验证了突变MEFs中蛋白的缺失。(来源:J:99997)
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A targeting construct was designed to insert a PGK-neo into exon 1, thereby eliminating 92% of the coding sequence. Western blot confirmed absence of protein in mutant MEFs. (J:99997)