大部分exon 4编码的大部分跨膜区域被替换了 neo抗药性基因和lacZ基因。通过 Western blot 验证了突变体中gamma亚基的缺失。(来源:J:99929)
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Most of exon 4, encoding most of the transmembrane domain, was replaced with a neomycin resistance gene and a lacZ gene. Absense of gamma subunits in mutants was confirmed by Western blot. (J:99929)