设计了一个靶向载体,用于在编码膜蛋白1和2的外显子中插入新霉素抗性基因座。突变脑样本的 Western blot 显示无蛋白表达。(来源:J:99813)
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A targeting vector was designed to insert a neomycin resistance cassette in the exon encoding transmembranes 1 and 2. Western blot of mutant brain samples confirmed absence of protein. (J:99813)