在pro-protein区域的第八外显子中插入了终止密码子和诺卡因抗性基因座,但使用针对细胞质区的抗体进行 Western blot 时未能检测到成熟蛋白。(来源:J:99692)
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A termination codon and neomycin resistance cassette were inserted into exon 8 in the pro-protein domain. Western blot using antisera to the cytoplasmic domain failed to detect mature protein. (J:99692)