一个新霉素抗性基因替换了包含起始密码子和EGF域N端半区域的exon 1和2。 Western blot结果显示突变体大脑中没有蛋白质表达(来源:J:99761)
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A neomycin resistance gene replaced exon 1 and 2, which encompass the start codon and N-terminal half of the EGF domain. Western blot confirmed absence of protein in mutant brains. (J:99761)