在所有三个ORF的第58个密码子后引入了终止密码子。一个新霉素抗性基因替换了编码DNA结合区域的序列。 Western blot分析证实了突变体的表达缺失。(来源:J:99185)
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Translation termination codons were introduced in all three ORFs after codon 58. A neomycin resistance gene replaced the sequence encoding the DNA binding domain. Western blot analysis confirmed lack of expression in mutants. (J:99185)