Thbs3^tm1Bst

在B外显子中插入了由loxP座引导的PGK-neo序列，该序列在 cassette 之前包含了一个框内终止密码子，通过Northern blot检测到突变肺部的RNA，证实了产生了无功能等位基因。 (Source: Northern blot of RNA from mutant lungs confirmed the generation of a null allele by the insertion of a PGK-neo flanked by loxP sites in exon B, incorporating an in-frame stop codon upstream from the cassette.)