在exon 4中插入了一个IRES-lacZ-neo插件,通过定量RT-PCR和 Western blot,我们确认了突变脑组织中没有转录物和蛋白质的存在。(来源:J:98804)
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An IRES-lacZ-neo cassette was inserted into exon 4. Quantitative RT-PCR and Western blot confirmed absence of transcript and protein in mutant brain samples. (J:98804)