设计了一个靶向元件,用于将2-4号外显子替换为诺卡因抗性基因。2号外显子编码膜蛋白区域。 Western blot分析在突变脾细胞中未检测到蛋白质(来源:J:98403)
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A targeting construct was designed to replace exons 2-4 with a neomycin resistance gene. Exon 2 encodes the transmembrane domain. Western blot analysis failed to detect protein in mutant splenocytes. (J:98403)