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A targeting vector containing a PGK-neo was inserted into exon 1, 30 bp downstream from the ATG. Northern and Western blot analyses confirmed disruption of transcript and protein, respectively, from exon 1. A transcript was found that appeared to result from the fusion of exon 2 to 403 bp from the 3' end of the intron instead of to the neo transcript. A truncated protein was also found at significantly reduced levels than wild-type. (J:98334)