在KIX域编码序列的上游插入了floxed neomycin选择 cassette,然后对包含KIX的exon进行了改造,将第650位的酪氨酸替换为异亮氨酸(Y650A),第654位的异亮氨酸替换为谷氨酰胺(A654Q),第658位的酪氨酸替换为异亮氨酸(Y658A)。随后通过短暂的Cre表达,移除了选择 cassette。突变产物的表达和稳定性保持正常。(来源:J:98329)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
核苷酸替换
--
1
13
8

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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