在BMMCs中,通过PGK-neo替换了包含第3外显子的1.7kb序列,该区域编码68-106位氨基酸, Western blot实验确认了目标区域没有检测到蛋白质(来源:J:97991)
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A PGK-neo replaced a 1.7 kb sequence including exon 3, the region encoding amino acids 68-106. Western blot of BMMCs confirmed absence of protein from the targeted region. (J:97991)