Tcra-J^tm2.3Krg

Tcra-Jtm2.1Krg ES cells (where the Trja49 promoter (Rr319) and gene segment were replaced with a loxP site flanked neomycin resistance gene cassette and the neo cassette was removed through subsequent cre-mediated recombination) were targeted with a vector replacing the TEA (T early alpha) promoter with a floxed neo cassette. The region between the loxP site in place of Trja49 and the upstream loxP site in place of the TEA promoter was deleted through subsequent cre-mediated recombination. This deletes segments Traj50-61 and the non-coding TEA exon. No gross deficiency was found in alphabeta TCR expression or alphabeta T cell development in mutant mice. (J:97736)