Tcra-J^tm2.2Krg

Tcra-Jtm2.1Krg ES cells (where the Trja49 promoter (Rr319) and gene segment were replaced with a loxP site flanked neomycin resistance gene cassette and the neo cassette was removed through subsequent cre-mediated recombination) were targeted with a vector replacing the TEA (T early alpha) promoter with a floxed neo cassette, which was removed through subsequent cre-mediated recombination. This allele lacks both the Jalpha49 and TEA promoters. No gross deficiency was found in alphabeta TCR expression or alphabeta T cell development in mutant mice. (J:97736)