在第10个内含子中插入了敲除的PGK-neo,产生了这种表型弱化的等位基因。 Western blot结果显示,突变巨噬细胞中该蛋白显著减少,大约只有野生型水平的10% (来源:J:97351)
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A floxed PGK-neo was inserted into intron 10 to create this hypomorphic allele. Western blot showed that protein was dramatically reduced in mutant macrophages to approximately 10% of wild-type levels. (J:97351)