在心脏中插入了NLS-lacZ和neo cassette,以替换3号外显子的部分区域以及4号和5号全外显子,编码的正是整个膜蛋白区域。通过 Western blot 分析,结果显示没有检测到蛋白质表达(来源:J:97365)
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An NLS-lacZ and a neo cassette were inserted to replace part of exon 3 and all of exons 4 and 5, sequence that encodes the entire transmembrane domain. Mutant hearts were analyzed by Western blot and demonstrated a lack of protein. (J:97365)