目标等位基因携带有frt标记的neo cassette和一个被floxed的exon 5。随后通过瞬时表达Flp重组酶,移除了neo cassette,导致exon 5被loxP-frt(上游)和loxP(下游)序列所 flank。在纯合小鼠大脑的 Western blot 检测中,显示了正常isoform谱和表达水平。(来源:J:96990)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count