在基因中插入了由loxP座标记的耐氨苄青霉素抗性序列,导致9c编码区域、上游的UTR和剪接点都被删除。 Western blot结果显示,这种变异体的大脑中没有检测到该蛋白(来源:J:97192)。
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A neomycin resistance flanked by loxP sites was inserted into the gene, resulting in the deletion of the entire 9c coding and the upstream UTR and splice sites. Western blot confirmed absence of protein from this isoform in mutant brains. (J:97192)