Cfl1tm1Wit小鼠与Cre-删除小鼠杂交,以删除exon 2和neo cassette,这导致了框架移位和立即的翻译终止。 Western blot结果显示突变胚胎的提取物中没有蛋白质(来源:J:96452)。
查看原文 参与反馈
Cfl1tm1Wit mice were crossed to Cre-deleter mice to delete exon 2 and the neo cassette. The deletion created a frame shift and immediate translational stop. Western blot confirmed absence of protein in mutant embryo lysates. (J:96452)