Nkd1^tm1Tko

Exons 5 and 6, containing 60 amino acids comprising most of the EF-hand-containing oxygen donation and ion-binding domains, were replaced with a PGK-neo. Exons 4 and 7 were spliced together and resulted in a mutant transcript and protein lacking exons 5 and 6, as shown by Northern blot analysis, RT-PCR and Western blot. (J:95901)