将1-4外显子替换为全长Ccnd2基因和一个PGK-neo后,通过对突变脾和肾的 Western blot 检测,证实了Ccnd1蛋白缺失以及Ccnd2蛋白的存在(来源:J:95994)
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Exons 1-4 were replaced by the full-length Ccnd2 cDNA and a PGK-neo. Western blot of mutant spleen and kidney confirmed the lack of Ccnd1 protein and presence of Ccnd2 protein. (J:95994)