在第一个外显子内插入了一个含有neo基因的基因陷阱 cassette,通过定量RT-PCR和 cerebellum 组织的 Western blot 分析,确认了RNA和蛋白质的缺失。(来源:J:157850)
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A gene trap cassette containing a neo gene was inserted within the first exon. Absence of RNA and protein was confirmed by quantitative RT-PCR and western blot analysis of cerebellum extracts. (J:157850)