通过LacZ和neo cassette,对基因编码区2到7(Coding exons 2 through 7)进行了靶向克隆。转录表达的缺失通过在脑组织提取物上进行RT-PCR得到了确认(参考文献J:103485, J:164681)。
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Coding exons 2 through 7 were targeted with a LacZ and neo cassette. The absence of transcript expression was confirmed by RT-PCR on brain extracts. (J:103485, J:164681)