在exon 1的起始密码子后插入了带有floxed标签的EGFP-neo基因。在发育和成年组织中显示了EGFP的表达。对晶状体提取物进行 Western blot 显示了突变体中无Hsf4蛋白的存在。(来源:J:95535)
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A floxed EGFP-neo was inserted in-frame after the start codon in exon 1. EGFP expression was demonstrated in developing and adult tissues. Western blot of lens extracts showed the lack of Hsf4 protein in mutants. (J:95535)