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中文
设计了一种靶向载体，旨在随后删除exon 2编码序列，并将人SMAD3的氨基端标签基因插入该位点。插入了三重重复的SV40polyA序列以保证转录终止，并带有条件性floxed PGK-neo。短暂的Cre表达剪除了条件性neo。通过RNA酶保护分析设计了探针来确认人基因组DNA的表达，突变组织中残留的内源性转录几乎不可检测。突变体中重组转录本的表达水平与野生型动物的内源水平相当。（来源：J:95374）
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
