整个编码区域被来自pSL1180载体的ATG-lacZ-PGK-neo cassette替换。 Western blot结果显示肝脏、脾脏、睾丸和胸腺中没有蛋白质表达(来源:J:204462)
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The entire coding region was replaced with an ATG-lacZ-PGK-neo cassette derived from the pSL1180 vector. Western blot analysis confirmed the asbence of protein expression in the liver, spleen, testis and thymus. (J:204462)