基因陷阱载体pGT1lxf插入在第18和19外显子之间。RT-PCR和 Western blot 分析证实了纯合MEFs中mTNA和蛋白表达的缺失。(来源:J:139829)
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The gene trap vector, pGT1lxf, inserted between exon 18 and exon 19. RT-PCR and western blot analysis confirm the absence of mTNA and protein expression in homozygous MEFs. (J:139829)