在exon 2下游插入了一个floxed neo cassette,然后在exon 4下游再插入了一个loxP位点。通过小鼠的生殖细胞中cre介导的重组,移除了neo cassette,使得exons 2到4都被留下了loxP标记。 (Source: The given text)
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A floxed neo cassette was inserted downstream of exon 2, and an additional loxP site was inserted downstream of exon 4. Germ line, cre mediated recombination was used to remove the neo cassette leaving exons 2 through 4 floxed.