一种新霉素抗性基因座替换了整个开放阅读框。通过定量PCR无法检测到mRNAQ,而来自纯合突变小鼠的提取物中也无法通过 Western blot 鉴定出蛋白质产品。(来源:J:131735)
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A neomycin resistance cassette replaced the entire open reading frame. mRNAQ cannot be found by quantitative PCR and protein product cannot be identified by Western blot in extracts from homozygous mutant mice. (J:131735)