Plce1^tm1Tkat

A PGK-neo was inserted to replace the 3' end of one of the four exons encoding the X domain. RT-PCR demonstrated the presence of a shortened transcript in mutant heart samples. Sequence analysis revealed a deletion of another upstream exon, presumably caused by exon skipping upon RNA splicing. The resulting allele contains an in-frame deletion of amino acids 1333 to 1408, corresponding to the amino terminal end of the catalytic X domain. (J:94952, J:97661)