在ALS2的首个编码外显子中,一个39bp的BamHI片段被lacZ基因和带有loxP座的筛选标记替换。qPCR和 Western blot的结果证实了该区域无转录和翻译活动。(来源:J:100336)
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A 39 bp BamHI fragment in the 1st coding exon of ALS2 was replaced with a lacZ gene and selection marker flanked with loxP sites. QPCR and Western blot analysis confirmed the absence of transcription and translation. (J:100336)