一段包括2/3 intron和大部分3号外显子的400bp片段被替换了新基因。3号外显子含有所需的ATP结合的赖氨酸残基。对突变体胸腺和脾脏的 Western blot 显示无相应蛋白存在。(来源:J:94685)
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A 400 bp fragment including the intron 2/exon 3 junction and most of exon 3 was replaced with a neo. Exon 3 contains the lysine residue required for ATP binding. Western blot of thymus and spleen from mutants confirmed a lack of protein. (J:94685)