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中文
设计了一个靶向载体，目标是在编码激酶域(413-444氨基酸段)外显子的区域插入loxP位点。当与表达Cre的质粒转染后，含floxed PGK-neo cassette的靶向元件被删除，外显子被留了下来。载体设计使得Cre介导的重组会引入相邻外显子的框架突变，阻止了功能蛋白的产生。免疫印迹确认了在Cre表达的ES细胞中未检测到野生型蛋白或由这个等位基因产生的任何截短蛋白。(来源：J:94680)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
