Del(11Cops3-Gid4)2Jrl

A deletion between Cops3 and Gid4 (previously, 4933439F18Rik) was engineered in ES cells by insertion of complementary partial Hprt-loxP constructs at each site. Insertion at Cops3 was specifically targeted whereas insertion in intron 3 of Gid4 was a random insertion brought about using a V15 retrovirus. Cre recombinase actived deleted the intervening region and reconstructed the Hprt minigene for selection purposes. Two additional, phenotypically identical, deletions were created in which the loxP-Hprt partial gene was inserted in intron 5 of Gid4 (MGI:3521943 and MGI:3521944). (J:94405)