Frs2^tm1Schl

Point mutations were introduced into the genomic sequence so that the encoded protein contained phenylalanine in place of tyrosine at amino acid positions 436 and 471, which disrupted the binding site for protein tyrosine phosphatase, non-receptor type 11 (also known as Shp2). A loxP-flanked neomycin resistance cassette was inserted into the fourth intron. Mice bearing this mutant allele were crossed to mice expressing Cre recombinase, under control of the human cytomegalovirus promoter/enhancer, to delete the neo cassette in the germline. Immunoblot analysis demonstrated similar levels of expression of mutant and wild-type protein in homozygous mutant and wild-type mice, respectively. (J:94724)