β-半乳糖苷酶的编码序列后面跟着一个PGK-neo cassette,它被插入在第180位残基之后的顺式位置。QRT-PCR和 Western blot 实验确认了没有转录和翻译产物存在。(来源:J:111469)
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The coding sequence of beta-galactosidase followed by a PGK-neo cassette was inserted in frame downstream of the sequence for residue 180. QRT-PCR and Western blot analysis confirmed the absence of transcription and translation products. (J:111469)