第二外显子被替换成了一种诺卡因抗性基因座。通过 Western blot 分析确认了没有蛋白质产物产生。这个等位基因不干扰反向排列的下游基因,它与第三外显子和部分内含子 2 有重叠。(来源:J:94752)
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Exon 2 was replaced with a neomycin resistance cassette. Absence of protein product was confirmed by Western blot analysis. This allele does not disrupt a downstream gene in reverse orientation that overlaps exon 3 and part of intron 2. (J:94752)