通过同源重组,PGK-neo基因替换了1号外显子的部分(1),2到6号外显子,以及7号外显子的部分,去除了编码序列的83%,包括了催化切点。 Western blot分析证实MEFs(J:94227)中没有蛋白表达。 (Source: J:94227)
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A PGK-neo gene replaced part of exon 1, exons 2 to 6, and part of exon 7 via homologous recombination, removing 83% of the coding sequence including the proteolytic catalytic site. Western blot analysis confirmed absence of protein in MEFs (J:94227)