通过同源重组,在内皮3区插入一个新 cassette,旁边带有loxP位点,同时在exon 9内也插入了另一个loxP,形成了一个neo cassette。在重组的ES细胞中表达Cre重组酶,导致4-9号外显子被删除。 Western blot检测在P0时的海马匀浆和培养10天的海马神经元中,证实了没有蛋白质的存在。(来源:J:93969)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S1/Sv-Oca2+ Tyr+ Kitl+
Targeted
基因内删除
--
1
6
9

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
No Data Found!
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