在替换为含有loxP位点的neo时,去除了1-12号外显子。随后通过与删除者小鼠杂交移除了neo。突变胸腺细胞的 Western 印迹结果显示完全没有蛋白质。(来源:J:94074)
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Exons 1-12 were removed upon replacement with a loxP flanked neo. The neo was subsequently removed by crossing with deleter mice. Western blot of mutant thymocytes indicated a complete lack of protein. (J:94074)