The gene trap vector PT1-ATG inserted in to a 5' exon in the reverse orientation. The vector contained the En2 splice acceptor site, lacZ, and PGK-neo. RNase protection analysis indicated that the integrated vector was expressed as a fusion protein, including 320 bp of Mospd3 combined with 23 bp of En2 intronic and 120 bp of En2 exonic sequence. (J:31729, J:47966)